Fluorescence Filters

Date:2019-06-25 Click:431

Our team designs and manufactures the most diverse fluorescent filters in the industry for biomedical fluorescence detection and high resolution requirements analysis systems such as PCR analyzers, POCTs, fluorescence microscopy, flow cytometry, etc. Welcome to the optimization of the fluorophores, using the superb coating process, the crosstalk coefficient of the fluorescence filter manufactured by Wendi in the multi-channel fluorescence test has reached a very low ideal level.

Product advantages

■ No wavelength drift – providing precise and stable center wavelengths;
■ High transmission rate – ensuring maximum signal collection efficiency;
■ High cut-off steepness – cut off the excitation light in the fluorescent emission band for better contrast;
■ High cut-off depth – ensures optical system background noise is minimized and signal to noise ratio is maximized;

General Specifications

Parameter

Value

Comment

Averaged Transmission

93%

Averaged over the passband

Blocking Depth

OD6

/

Angle of Incidence

0°±2°

/

Diameter/mm

12, 12.7, 15.0, 25.0, 25.4

Accept custom size

Thickness/mm

1~6

Mounted in black-anodized aluminum ring

Clear Aperture/mm

80%

/

Surface Quality

60-40 Scratch-Dig

/

Reliability & Durability

Environmental: MIL-STD-810F, Physical: MIL-C-48497A

Orientation

The arrow on the ring indicates the recommended direction of propagation of light.

Typical Fluorescence Filter Sets

Excitation Filter

Emission Filter

Blocking Range/nm

Blocking Depth

365/10

615/20

200-800

OD6

470/30

525/20

200-800

OD6

485/20

520/20

200-800

OD6

525/20

564/20

200-800

OD6

530/20

565/22

200-800

OD6

543/20

584/20

200-800

OD6

571/20

612/20

200-800

OD6

585/20

610/20

200-800

OD6

635/30

680/20

200-800

OD6

655/40

716/40

200-900

OD6

710/40

810/50

200-1000

OD6

Note: 365/10 indicates that the central wavelength is 365 nm and FWHM (the full width half maximum) is 20 nm.



Fluorescence Filter Sets for qPCR and Measured Spectrum
Channel 1: FAM/SYBR Green

Note: excitation filter: BP470/30, dichroic mirror: DLWP500, emission filter: BP525/20.

Channel 2: HEX/VIC/TET/JOE/CY3/TAMRA


Note: excitation filter: BP525/20, dichroic mirror: DLWP565, emission filter: BP564/20.
Channel 3: ROX/TexRed

Note: excitation filter: BP571/20, dichroic mirror: DLWP586, emission filter: BP612/20.

Channel 4: CY5/Quasar-670

Note: excitation filter: BP635/30, dichroic mirror: DLWP665, emission filter: BP682/20.
Channel 5: CY5.5/ Alexa Fluor® 680/ Alexa Fluor® 700


Note: excitation filter: BP655/40, dichroic mirror: DLWP685, emission filter: BP716/40.